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E-GEOD-73376 - DGCR8 acts as a novel adaptor for the exosome complex to degrade double-stranded structured RNAs

Status
Released on 10 December 2015, last updated on 20 December 2015
Organism
Homo sapiens
Samples (11)
Protocols (7)
Description
The Microprocessor complex (DGCR8/Drosha) is required for microRNA (miRNA) biogenesis but also binds and regulates the stability of several types of cellular RNAs. Of particular interest, DGCR8 controls the stability of mature small nucleolar RNA (snoRNA) transcripts independently of Drosha, suggesting the existence of alternative DGCR8 complex/es with other nucleases to process a variety of cellular RNAs. Here, we found that DGCR8 co-purifies with subunits of the nuclear exosome, preferentially associating with its hRRP6-containing nucleolar form. Importantly, we demonstrate that DGCR8 is essential for the recruitment of the exosome to snoRNAs and to human telomerase RNA. In addition, we show that the DGCR8/exosome complex controls the stability of the human telomerase RNA component (hTR/TERC). Altogether, these data suggests that DGCR8 acts as a novel adaptor to recruit the exosome complex to structured RNAs and induce their degradation. [i] Examination of the RNA binding profile of hRRP6 (also known as EXOSC10) via iCLIP. [ii] HeLa cells were transiently depleted of hRRP6 or DGCR8 using siRNAs. For a control an non-targetting (siNon) siRNA was used. Three biological replicates of each samples were sent for RNA sequencing.
Experiment type
RNA-seq of coding RNA 
Contacts
Mireya Plass <mplass@binf.ku.dk>, Javier F Cáceres, Philippe Gautier, Ross A Cordiner, Sara Macías
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-73376.idf.txt
Sample and data relationshipE-GEOD-73376.sdrf.txt
Processed data (1)E-GEOD-73376.processed.1.zip
Additional data (1)E-GEOD-73376.additional.1.zip
Links