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E-GEOD-73146 - Gene expression over the course of xylem tracheary element formation in cell suspension cultures of Arabidopsis thaliana

Status
Released on 18 September 2015, last updated on 10 December 2015
Organism
Arabidopsis thaliana
Samples (27)
Array (1)
Protocols (7)
Description
Transcriptomic analysis of gene expression during the differentiation of cell suspension cultures into tracheary elements using the biological system published by Pesquet et al., Current Biology (2010): tracheary element differentiation was triggered by externally supplying hormone-free habituated cell suspension cultures of Arabidopsis thaliana Col-0 with auxin, cytokinin and epibrassinolides; RNA samples extracted from 3 independent time-courses every 12h from 0h to 4 days were analyzed using ATH1 Arabidopsis Affymetrix micro-array Time-course design during tracheary element differentiation (3 independepent time-course were harvested from initiation 0h to 4 days every 12h) - total of 27 samples corresponding to the 3 replicates of 9 time point time-courses - gene expression was monitored along the time-course compared to 0h
Experiment type
transcription profiling by array 
Contacts
Edouard Pesquet
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-73146.idf.txt
Sample and data relationshipE-GEOD-73146.sdrf.txt
Raw data (1)E-GEOD-73146.raw.1.zip
Processed data (1)E-GEOD-73146.processed.1.zip
Array designA-AFFY-2.adf.txt
Links