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E-GEOD-73000 - The crystal structure of the NHL domain in complex with RNA reveals the molecular basis of Drosophila Brain tumor-mediated gene regulation
Released on 14 September 2015, last updated on 20 September 2015
Here, we analyze the RNA-binding preferences of several NHL containing RNA-binding proteins (BRAT, NCL-1, TRIM71, WECH, LIN-41, TRIM56, and MEI-P26) using RNAcompete. In the RNAcompete assay, purified GST-tagged RNA-binding protein is incubated with an excess of RNA pool and bound RNA from individual pulldown experiments are directly labeled and hybridized to a custom Agilent 244K microarray.
Debashish Ray <email@example.com>, Gunter Meister, Inga Loedige, Janosch Hennig, Julia C Engelmann, Kate B Cook, Leonhard Jakob, Mathias Stotz, Michael Krahn, Nora Treiber, Quaid D Morris, Thomas Treiber, Timothy R Hughes