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E-GEOD-73000 - The crystal structure of the NHL domain in complex with RNA reveals the molecular basis of Drosophila Brain tumor-mediated gene regulation

Status
Released on 14 September 2015, last updated on 20 September 2015
Organism
synthetic construct
Samples (7)
Array (1)
Protocols (5)
Description
Here, we analyze the RNA-binding preferences of several NHL containing RNA-binding proteins (BRAT, NCL-1, TRIM71, WECH, LIN-41, TRIM56, and MEI-P26) using RNAcompete. In the RNAcompete assay, purified GST-tagged RNA-binding protein is incubated with an excess of RNA pool and bound RNA from individual pulldown experiments are directly labeled and hybridized to a custom Agilent 244K microarray.
Experiment type
other 
Contacts
Debashish Ray <debray1@gmail.com>, Gunter Meister, Inga Loedige, Janosch Hennig, Julia C Engelmann, Kate B Cook, Leonhard Jakob, Mathias Stotz, Michael Krahn, Nora Treiber, Quaid D Morris, Thomas Treiber, Timothy R Hughes
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-73000.idf.txt
Sample and data relationshipE-GEOD-73000.sdrf.txt
Raw data (1)E-GEOD-73000.raw.1.zip
Processed data (1)E-GEOD-73000.processed.1.zip
Array designA-GEOD-16119.adf.txt
Links