E-GEOD-7101 - Transcription profiling by array of human p53+ and p53- fibroblasts treated with arsenite
Released on 11 November 2007, last updated on 5 October 2015
Microarray data from G2-synchronized p53(+) and p53(-) fibroblasts before and after 3 h release from cell cycle blockade in the presence of 5 uM sodium arsenite. Experiment Overall Design: Cells expressing p53 from a tet-off regulated construct were synchronized in G2 with a two-step procedure using 24 h aphidicolin treatment for initial G1 synchronization and 12 h of Hoechst 33342 to effect a G2 blockade. During Hoechst treatment, tetracycline was added to suppress p53 in half the cultures. Cells were then released into media containing 5 µM sodium arsenite and the appropriate concentration of tetracycline to maintaining p53 expression. mRNAs were collected at 0 h and 3 h after release from G2 synchrony.
transcription profiling by array, co-expression, compound treatment
Exit from arsenite-induced mitotic arrest is p53 dependent. McNeely SC, Xu X, Taylor BF, Zacharias W, McCabe MJ Jr, States JC. , Europe PMC 16966095