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E-GEOD-71001 - Transcription profiling by array of Arabidopsis roots treated with salt from bzip1, bzip53 double knockout

Status
Released on 2 September 2015, last updated on 25 November 2015
Organism
Arabidopsis thaliana
Samples (24)
Array (1)
Protocols (7)
Description
Soil salinity increasingly causes crop losses worldwide. Although roots are the primary targets of salt stress, the signaling networks that facilitate metabolic reprogramming to induce stress tolerance are less understood than those in leaves. Here, a combination of transcriptomic and metabolic approaches was performed in salt-treated Arabidopsis thaliana roots, which revealed that the group S1 basic leucine zipper transcription factors bZIP1 and bZIP53 reprogram primary C- and N-metabolism. In particular, gluconeogenesis and amino acid catabolism are affected by these transcription factors. Importantly, bZIP1 expression reflects cellular stress and energy status in roots. In addition to the well-described abiotic stress response pathway initiated by the hormone abscisic acid (ABA) and executed by SnRK2 (Snf1-RELATED-PROTEIN-KINASE2) and AREB-like bZIP factors, we identify a structurally related ABA-independent signaling module consisting of SnRK1s and S1 bZIPs. Crosstalk between these signaling pathways recruits particular bZIP factor combinations to establish at least four distinct gene expression patterns. Understanding this signaling network provides a framework for securing future crop productivity. RNA from roots from Control and salt treated hydroponically grown seedlings were extracted and subjected to microarray analysis
Experiment type
transcription profiling by array 
Contacts
Citation
Crosstalk between Two bZIP Signaling Pathways Orchestrates Salt-Induced Metabolic Reprogramming in Arabidopsis Roots. Hartmann L, Pedrotti L, Weiste C, Fekete A, Schierstaedt J, Gottler J, Kempa S, Krischke M, Dietrich K, Mueller MJ, Vicente-Carbajosa J, Hanson J, Droge-Laser W. , PMID:26276836
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-71001.idf.txt
Sample and data relationshipE-GEOD-71001.sdrf.txt
Raw data (1)E-GEOD-71001.raw.1.zip
Processed data (1)E-GEOD-71001.processed.1.zip
Array designA-AFFY-2.adf.txt
Links