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E-GEOD-70202 - Transcriptomic impact of AWR5 effector expression in S. cerevisiae

Status
Released on 31 December 2015, last updated on 18 January 2016
Organism
Saccharomyces cerevisiae
Samples (12)
Array (1)
Protocols (5)
Description
The type III effector (T3E) AWR5 from the phytopathogen Ralstonia solanacearum causes disease in many plants relevant in agriculture. Heterologous expression in yeast of AWR5 from an integrated tetO promoter caused strong growth inhibition. In this series of experiments we investigate the possible changes in the transcriptional profile in a time-course (2, 4 and 6 h) response to AWR5 production. Yeast strains were grown overnight in rich YPD medium with doxycycline 15 µg/ml (repressing conditions), then normalized to OD600=0.05 and grown resumed in YPD+dox (non-inducing conditions) or YPD (inducing conditions). Samples were taken at 2, 4 or 6 hours for each condition. Two biological replicates were made for each time point.
Experiment type
transcription profiling by array 
Contacts
JOAQUIN ARINO <joaquin.arino@uab.es>, Crina Popa, Joaquin Ariño, Laura Tatjer, M Tabuchi, Marc Valls, Núria S Coll, Sergio Gil
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-70202.idf.txt
Sample and data relationshipE-GEOD-70202.sdrf.txt
Raw data (1)E-GEOD-70202.raw.1.zip
Processed data (1)E-GEOD-70202.processed.1.zip
Array designA-GEOD-10039.adf.txt
Links