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E-GEOD-70168 - The deafness gene DFNA5 induces programmed cell death through mitochondria and MAPK-related pathways [BY4741]

Status
Released on 24 June 2015, last updated on 27 June 2015
Organism
Saccharomyces cerevisiae
Samples (16)
Array (1)
Protocols (7)
Description
The function of DFNA5 remained unknown for a long time, but previous functional studies by Op de Beeck et al. (2011) revealed that DFNA5 induces a growth defect in mutDFNA5- transfected HEK293T cells, as well as other cells, leading to PCD (Op de Beeck et al., 2011). The cell death-inducing capacity of DFNA5 was not only restricted to human cell lines, but was also observed in the yeast model Saccharomyces cerevisiae (Van Rossom et al., 2012). This inspired us to perform a transcriptomic analysis using two different model organisms (mammalian, HEK293T, and yeast, S.cerevisiae) to further elucidate the mechanisms related to DFNA5. Comparison of 2 transfected yeast cell populations in 2 different growth phases. In total, 4 comparisons were made, each comparison was done in duplo by a colourflip. Every conditions is represented through 2 biol reps.
Experiment type
transcription profiling by array 
Contacts
Ken Op de Beeck <ken.opdebeeck@uantwerpen.be>, Guy Van Camp, Joris Winderinckx, Sofie Van Rossom, Vesna Hristovska
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-70168.idf.txt
Sample and data relationshipE-GEOD-70168.sdrf.txt
Raw data (1)E-GEOD-70168.raw.1.zip
Processed data (1)E-GEOD-70168.processed.1.zip
Array designA-GEOD-16244.adf.txt
Links