E-GEOD-6974 - Active chromatin domains are defined by acetylation islands revealed by genome-wide mapping
Released on 5 February 2007, last updated on 27 March 2012
The identity and developmental potential of a human cell is specified by its epigenome that is largely defined by patterns of chromatin modifications including histone acetylation. Here we report high-resolution genome-wide mapping of diacetylation of histone H3 at Lys 9 and Lys 14 in resting and activated human T cells by genome-wide mapping technique (GMAT). Our data show that high levels of the H3 acetylation are detected in gene-rich regions. The chromatin accessibility and gene expression of a genetic domain is correlated with hyperacetylation of promoters and other regulatory elements but not with generally elevated acetylation of the entire domain. Islands of acetylation are identified in the intergenic and transcribed regions. The locations of the 46,813 acetylation islands identified in this study aresignificantly correlated with conserved noncoding sequences (CNSs) and many of them are colocalized with known regulatory elements in T cells. TCR signaling induces 4045 new acetylation loci that may mediate the global chromatin remodeling and gene activation. We propose that the acetylation islands are epigenetic marks that allow prediction of functional regulatory elements. Keywords: SAGE-ChIP Using GMAT, we determined the genome-wide distribution of K9/K14 diacetylated histone H3 in resting and activated human T cells.
Keji Zhao <email@example.com>, S Cuddapah, Tae-Young Roh
Active chromatin domains are defined by acetylation islands revealed by genome-wide mapping. Roh TY, Cuddapah S, Zhao K. , Europe PMC 15706033