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E-GEOD-69686 - Post-natal age is a critical determinant of the neonatal host response to sepsis

Status
Released on 6 July 2015, last updated on 20 August 2015
Organism
Homo sapiens
Samples (150)
Array (1)
Protocols (5)
Description
Neonates manifest a unique host response to sepsis even among other children. Preterm neonates may experience sepsis soon after birth or during often protracted birth hospitalizations as they attain physiologic maturity. We examined the transcriptome using genome-wide expression profiling on prospectively collected peripheral blood samples from infants evaluated for sepsis within 24 hours after clinical presentation. Simultaneous plasma samples were examined for alterations in inflammatory mediators. Group designation (sepsis or uninfected) was determined retrospectively based on clinical exam and laboratory results over the next 72 hours from the time of evaluation. Unsupervised analysis showed the major node of separation between groups was timing of sepsis episode relative to birth (early, <3 days or late, >3 days). Principal component analyses revealed significant differences between patients with early or late sepsis despite the presence of similar key immunologic pathway aberrations in both groups. Unique to neonates, the uninfected state and host response to sepsis is significantly affected by timing relative to birth. Future therapeutic approaches may need to be tailored to the timing of the infectious event based on post-natal age. We used human microarrays to detail the molecular profile of the events that occur following sepsis in hospitalized neonates Please note that 'uninfected chorio' represents babies who were not infected but had chorioamnionitis exposure
Experiment type
transcription profiling by array 
Contacts
M Cecilia Lopez <mclopez@ufl.edu>, Hector R Wong, Henry V Baker, James L Wynn, Lyle L Moldawer, M C Lopez, Patrick Lahni, Ricardo Ungaro, Scott O Guthrie
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-69686.idf.txt
Sample and data relationshipE-GEOD-69686.sdrf.txt
Raw data (19)Click to browse raw data
Processed data (1)E-GEOD-69686.processed.1.zip
Array designA-GEOD-20292.adf.txt
Links