E-GEOD-6907 - Transcription profiling by array of human HepG2 cells after treatment with 2,3-Dimethoxy-1,4-naphthoquinone, N-nitrosodimethylamine, phenol and six heavy metals
Released on 8 April 2009, last updated on 30 April 2015
From the results of gene expression analyses of HepG2 under the exposure of 2,3-Dimethoxy-1,4-naphthoquinone (DMNQ), N-nitrosodimethylamine (DMN), phenol and six heavy metals We showed that biological action of six heavy metals were clearly related to that of DMNQ and distinguishable from the other chemicals. These results suggest that oxidative stress is major apparent biological action of high dose heavy metals, supporting the previous reports. Experiment Overall Design: Using Affymetrix HG-Focus arrays, we compared the gene expression patterns of Hep G2 cells induced by six heavy metals (As, Cd, Ni, Sb, Hg or Cr) with that of DMNQ, DMN or phenol, and evaluated the toxicities of these heavy metals.
transcription profiling by array, compound treatment design
Classification of heavy-metal toxicity by human DNA microarray analysis. Koji Kawata, Hiroyuki Yokoo, Ryuhei Shimazaki, Satoshi Okabe.