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E-GEOD-68996 - Expression analysis of Listeria monocytogenes LO28 delta-lhrC1-5 mutant and LO28 wt

Status
Released on 19 May 2015, last updated on 24 May 2015
Organism
Listeria monocytogenes LO28
Samples (12)
Array (1)
Protocols (7)
Description
Investigation of whole genome gene expression level changes in Listeria monocytogenes LO28 delta-lhrC1-5 mutant, compared to the wild type strain. The lhrC1-5 genes encode the regulatory sRNAs LhrC1-5. The microarray studied the gene expression of unstressed cells and cells exposed to cefuroxime for 30 min. The lhrC1-5 mutant employed in this study is further described in Sievers et al. (2014) A multicopy sRNA of Listeria monocytogenes regulates expression of the virulence adhesin LapB. Nucleic Acids Res. 42:9383-98. A twelve chip study using RNA extracted from 6 different cultures of L. monocytogenes LO28 delta-lhrC1-5 (three unstressed and three stressed cultures) and 6 different cultures of L. monocytognes LO28 wild-type (three unstressed cultures and three stressed cultures).
Experiment type
transcription profiling by array 
Contacts
Birgitte Haar Kallipolitis <geo@ncbi.nlm.nih.gov>, Birgitte H Kallipolitis, Susanne Sievers
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-68996.idf.txt
Sample and data relationshipE-GEOD-68996.sdrf.txt
Raw data (1)E-GEOD-68996.raw.1.zip
Processed data (1)E-GEOD-68996.processed.1.zip
Array designA-GEOD-18538.adf.txt
Links