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E-GEOD-68742 - Gene expression signatures in GIST-T1 cells transfected with a miR-335 mimic molecule

Status
Released on 25 May 2015, last updated on 30 May 2015
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (7)
Description
To clarify the effect of miRNAs, we carried out a gene expression microarray analysis using GIST-T1 cells transfected with a miR-335 mimic or a negative control. We found that 1,095 probe sets (853 unique genes) were downregulated (>1.5-fold) by ectopic miR-335 expression. GIST-T1 cells were transfected with a mirVana miR-335 mimic (Ambion) or a mirVana miRNA mimic Negative Control #1 (Ambion). Forty-eight hours after transfection, total RNA extraction was carried out, and gene expression signatures were analyzed.
Experiment type
transcription profiling by array 
Contacts
Hiromu Suzuki, Takeshi Niinuma
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-68742.idf.txt
Sample and data relationshipE-GEOD-68742.sdrf.txt
Raw data (1)E-GEOD-68742.raw.1.zip
Processed data (1)E-GEOD-68742.processed.1.zip
Array designA-GEOD-16699.adf.txt
Links