E-GEOD-6858 - Transcription profiling by array of lungs from mice mutant for RAG after challenge with ovalbumin
Released on 6 November 2007, last updated on 30 April 2015
Experimental asthma was induced in BALB/c mice by sensitization and challenge with the allergen ovalbumin. Control groups received PBS. To investigate the innate immune component of experimental asthma, we also analyzed recombinase activating gene (RAG) deficient mice following exposure to ovalbumin and control PBS Experiment Overall Design: Each experimental group was exposed to ovalbumin or control PBS. At day 22, 1 day after completion of the allergen exposure, whole lungs were harvested, total mRNA prepared, and gene expression determined by hybridization to Affymetrix microarrays.
transcription profiling by array, co-expression, compound treatment, individual genetic characteristics
Hubs in biological interaction networks exhibit low changes in expression in experimental asthma. Xin Lu, Vipul V Jain, Patricia W Finn, David L Perkins.