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E-GEOD-68298 - Expression data from aging study on mouse spermatogonial stem/progenitor cell population

Status
Released on 24 May 2015, last updated on 30 May 2015
Organism
Mus musculus
Samples (16)
Array (1)
Protocols (7)
Description
In mouse, spermatogonial stem/progenitor cells are the progenitor cell which develop to mature sperms through a series of mitotic and meiotic divisions and differentiation. Gfra1 is an established surface marker for mouse spermatogonial stem/progenitor cells. In this study, we used a transcriptomic approach to investigate the effect of aging on Gfra1-positive and -negative populations of mouse male germ cells. Spermatogonial stem/progenitor cells were isolated from testes of mice at ages 6-day, 21-day, 60-day, and 8-months by magnetic assisted cell sorting (MACS) technique using Gfra1 marker. Transcriptomes among different ages and Gfra1 status were compared.
Experiment type
transcription profiling by array 
Contacts
Alfred Chun Shui LUK <alfredluk@cuhk.edu.hk>, Jiji Jiang, Maria Kokkinaki, Marie-Claude Hofmann, Martin Dym, Nady Golestaneh, Tin-Lap Lee, Wai-Yee Chan, Zuping He
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-68298.idf.txt
Sample and data relationshipE-GEOD-68298.sdrf.txt
Raw data (1)E-GEOD-68298.raw.1.zip
Processed data (1)E-GEOD-68298.processed.1.zip
Array designA-AFFY-45.adf.txt
Links