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E-GEOD-6814 - Transcription profiling of monkey bone marrow-derived mesenchymal stem cells to examine the effect of age

Status
Submitted on 21 January 2007, released on 15 June 2008, last updated on 10 June 2011
Organism
Macaca mulatta
Samples (11)
Array (1)
Protocols (6)
Description
The objective of this study was to elucidate age-related differences in gene expression profiles of rhesus monkey bone marrow-derived mesenchymal stem cells (rhMSC) obtained from fetal, infant, and adult donors relevant to their growth and other properties. Although a high degree of similarity was observed in the rhMSC gene expression profiles when comparing the three age groups, significant differences were found that strongly parallel gene expression profiles of human MSC. The potential functional relevance of differential gene expression was most apparent when comparing fetal and adult rhMSC transcript profiles. Overall, the observed gene expression profiles are consistent with a loss of rhMSC pluripotency and proliferative capacity with advancing donor age. In addition, these data highlight the importance of use of non-human primates as a model system for studying the properties of human stem cells. Experiment Overall Design: A total of eleven bone marrow-derived mesenchymal stem cell cultures were established from rhesus monkeys of diverse ages (ranging from gestation day 120 to 8 year old adults). Total RNA was harvested from these cultures and gene expression analyses were conducted using oligonucleotide microarrays. The expression profiles of a subset of genes was confirmed by qRT-PCR analysis. A detailed manuscript that details the results of our studies is currently under review.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-6814.idf.txt
Sample and data relationshipE-GEOD-6814.sdrf.txt
Raw data (1)E-GEOD-6814.raw.1.zip
Processed data (1)E-GEOD-6814.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-6814.eSet.r
Links