E-GEOD-6802 - Transcription profiling by array of human bronchial epithelial cells infected with Pseudomonas aeruginosa, Staphylococcus aureus or respiratory syncytial virus

Status
Released on 11 November 2007, last updated on 30 April 2015
Organism
Homo sapiens
Samples (11)
Array (1)
Protocols (5)
Description
Bronchial epithelial cells represent the first line of defense against invading airborne pathogens. They are important contributors to innate mucosal immunity and provide a variety of anti-microbial effectors. To investigate the role of epithelial cells upon infection of airway pathogens, we stimulated BEAS-2B cells for 4 h with UV-inactivated bronchial pathogens including Staphylococcus aureus, Pseudomonas aeruginosa and Respiratory Syncitial Virus (RSV) that among other receptors can strongly activate TLR2, TLR4 and TLR3, respectively. Experiment Overall Design: All conditions were done in triplicates except for Staphylococcus aureus, were two replicates were done. As a control, unstimulated BEAS-2B were used. Altogether 11 arrays were hybridized.
Experiment types
transcription profiling by array, disease state design
Contact
Citation
Differential recognition of TLR-dependent microbial ligands in human bronchial epithelial cells. Anja K Mayer, Mario Muehmer, Jörg Mages, Katja Gueinzius, Christian Hess, Klaus Heeg, Robert Bals, Roland Lang, Alexander H Dalpke.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-6802.idf.txt
Sample and data relationshipE-GEOD-6802.sdrf.txt
Raw data (1)E-GEOD-6802.raw.1.zip
Processed data (1)E-GEOD-6802.processed.1.zip
Array designA-AFFY-37.adf.txt
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