Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-67246 - RNAi of TbNMD3 in procyclic form Trypanosoma brucei lines
Released on 28 April 2015, last updated on 2 May 2015
Trypanosoma brucei brucei
A procyclic form Trypansome brucei RNAi line (PTT parental line, transfected with pALC14 incorporating a TbNMD3 gene fragment) capable of inducing depletion of TbNMD3 was analysed for mRNA expression by RNAseq Cells were grown for 72 hours in culture; RNAi was induced in cells by the addition of 1 microgram/ml of tetracycline
RNA-seq of coding RNA
Alasdair Ivens <firstname.lastname@example.org>, Alasdair C Ivens, Keith Matthews, Melanie Buhlmann
NMD3 regulates both mRNA and rRNA nuclear export in African trypanosomes via an XPOI-linked pathway. Bï¿½hlmann M, Walrad P, Rico E, Ivens A, Capewell P, Naguleswaran A, Roditi I, Matthews KR.