normalization data transformation protocol
Basecalls performed using CASAVA 1.8.2 Sequenced reads were masked for sequencing adapters and mapped to the reference genome using CASAVA 1.8.2 Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using CASAVA 1.8.2 Genome_build: hg19 Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each sample. Columns are: Chromosome, Start location, Stop location, Gene, RPKM, base count.
nucleic acid library construction protocol
TriZol + RNeasy mini Kit (Qiagen, #74106) TruSeq RNA Stranded Sample Preparation (Illumina)