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E-GEOD-66828 - Amydala transcriptome changes in germ-free mice

Status
Released on 9 July 2015, last updated on 19 August 2015
Organism
Mus musculus
Samples (12)
Protocols (2)
Description
We sequenced mRNA from 12 samples extracted from mouse amygdala tissue to generate the first amygdala-specific murine transcriptome for germ-free mice (GF), conventionally raised controls (CON) and germ-free mice that have been colonized with normal microbiota from postnatal day 21 (exGF). Equal amounts of RNA from two to three animals were pooled to yield 4 samples per group (CON, GF, and exGF). Pairwise comparisons for CONvsGF, CONvsexGF, GFvsexGF were performed using DESeq2.
Experiment type
RNA-seq of coding RNA 
Contacts
Alan E Hoban, Feargal Ryan, Gerard Clarke, John F Cryan, Marcus Claesson, Roman M Stilling, Timoth G Dinan
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-66828.idf.txt
Sample and data relationshipE-GEOD-66828.sdrf.txt
Processed data (1)E-GEOD-66828.processed.1.zip
Additional data (1)E-GEOD-66828.additional.1.zip
Links