Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-66488 - Characterization of tumor extracellular vesicle RNA cargo

Status
Released on 5 March 2015, last updated on 6 March 2015
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (5)
Description
Comparative RNA profiling between tumor cells and their secreted extracellular vesicles. Results revealed enrichment in genes involved in cellular migration and metastasis in extracellular vesicles, in agreement with their role as mediators of tumor progression. Mice were orthotoplically transplanted with MDA-MB-231 Breast Adenocarcinoma cells. Cells and extracellular vesicles (EVs) from the resulting tumors were isolated. EVs were characterized by electron microscopy and Nanoparticle Tracking Analysis before total RNA isolation for comparative analysis with cellular RNA. Three biological replicates were analyzed in (technical) duplicate.
Experiment type
transcription profiling by array 
Contacts
Jacco Van Rheenen <j.vanrheenen@hubrecht.eu>, Alwin Kamermans, Anoek Zomer, Carrie Maynard, Elzo de Wit, Evelyne Beerling, Frederik Verweij, Jacco van Rheenen, Jordi Berenguer, Michiel Pegtel, Raymond Schiffelers, Ronny Schäfer, Saskia I Ellenbroek, Thomas Würdinger
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-66488.idf.txt
Sample and data relationshipE-GEOD-66488.sdrf.txt
Raw data (1)E-GEOD-66488.raw.1.zip
Processed data (1)E-GEOD-66488.processed.1.zip
Additional data (1)E-GEOD-66488.additional.1.zip
Array designA-GEOD-10558.adf.txt
Links