Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-66483 - Expression data from differentiating mouse embryonic stem cells wild type and lacking functional Pax7 gene

Status
Released on 26 February 2016, last updated on 7 March 2016
Organism
Mus musculus
Samples (18)
Array (1)
Protocols (7)
Description
Myogenic differentiation relies on Pax7 function. We used embryonic stem cells lacking functional Pax7 to follow its role in derivation of skeletal myoblasts. Microarray analysis allowed us to compare transcriptomes of undifferentiated and differentiating embryonic stem cells of two genotypes, i.e. Pax7+/+ and Pax7-/- at day 7 and 21 of culture. Undifferentiated mouse embryonic stem cells, embryiod bodies at day 7, and embryoid body outgrowths at day 21 of differentiation were collected for RNA extraction and hybridization on Affymetrix microarrays.
Experiment type
transcription profiling by array 
Contacts
Areta Magda Czerwinska <aretaczerwinska@biol.uw.edu.pl>, Anna Fogtman, Areta M Czerwinska, Iwona Grabowska, Joanna Bem, Karolina Archacka, Maria A Ciemerych, Marta Koblowska, Roksana Iwanicka
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-66483.idf.txt
Sample and data relationshipE-GEOD-66483.sdrf.txt
Raw data (1)E-GEOD-66483.raw.1.zip
Processed data (1)E-GEOD-66483.processed.1.zip
Array designA-GEOD-17400.adf.txt
Links