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E-GEOD-65675 - LOXL2 dependent genes in RA induced mES differentiation

Status
Released on 21 December 2015, last updated on 26 December 2015
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (5)
Description
Our results indicate that oxidation of TAF10 by LOXL2 induces its release from its promoters, leading to a block in TFIID-dependent gene transcription. Since TFIID complex is crucial for the expression of Nanog, Klf4, Sox2 and Oct4 and for maintaining the pluripotent state of embryonic stem cells, TAF10 oxidation by LOXL2 leads to inactivation of the pluripotency genes and a loss of pluripotent capacity in embryonic stem cells. Moreover, in vivo results demonstrate an essential role of LOXL2 in neural differentiation during zebrafish development: in the absence of LOXL2 the neural progenitor gene Sox2 is aberrantly overexpressed and neural differentiation is impaired. 12 samples were analyzed: mES transduced with control shRNA (n=3); mES transduced with LOXL2 shRNA (n=3), ES transduced with control shRNA and treated with RA (n=3); ES transduced with LOXL2 shRNA and treated with RA (n=3)
Experiment type
transcription profiling by array 
Contacts
Citation
LOXL2 Oxidizes Methylated TAF10 and Controls TFIID-Dependent Genes during Neural Progenitor Differentiation. Iturbide A, Pascual-Reguant L, Fargas L, Cebri� JP, Alsina B, Garc�a de Herreros A, Peir� S. , PMID:25959397
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-65675.idf.txt
Sample and data relationshipE-GEOD-65675.sdrf.txt
Raw data (1)E-GEOD-65675.raw.1.zip
Processed data (1)E-GEOD-65675.processed.1.zip
Array designA-GEOD-16570.adf.txt
Links