E-GEOD-6548 - Transcription profiling of human estrogen-dependent model of breast cancer created by transformation of normal mammary epithelial cells
Submitted on 16 December 2006, released on 14 June 2008, last updated on 27 March 2012
This study was performed to check that ESR1 and BMI1 are biologically active after lentiviral transduction of primary human mammary epithelial cells (HMECs) with lentiviral vectors expressing ESR1 and BMI1 from the human PGK promoter. ESR1 targets like PGR, PRLR and GREB1, but not TFF1 and XBP1, were induced by estradiol in the ESR1-expressing cells. BMI1 targets like BMI1, NEFL and CCND2 were repressed in the BMI1-expressing cells. BMI1 suppressed genes associated with squamous and neural differentiation in the ESR1 plus BMI1-expressing cells. Experiment Overall Design: Infect with lentiviruses 24 hours after reduction mammmoplasty. Grow cells in human mammosphere medium: Hepes-buffered DMEM/F12 supplemented with 20 ng/ml EGF, 1x B-27 (GIBCO) plus 1 nM 17-β-estradiol or 1 uM fulvestrant (ICI 182,780). Experiment Overall Design: Lentiviral vectors: pSD-82, ESR1; pSD-84, BMI1; pSD-86, gusA.
transcription profiling by array, unknown experiment type
An oestrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells. Stephan Duss, Sylvie André, Anne-Laure Nicoulaz, Maryse Fiche, Hervé Bonnefoi, Cathrin Brisken, Richard D Iggo. Breast Cancer Res 9(3):R38 (2007)