E-GEOD-6540 - Transcription profiling of mouse olfactory epithelium of Lip-C-treated vs. Lip-O-treated controls

Status
Submitted on 15 December 2006, released on 14 June 2008, last updated on 10 June 2011
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (13)
Description
Microarray analysis of gene expression in the olfactory epithelium of macrophage depleted mice to study the role of macrophages in regulating neurodegeneration, neuroprotection, and neurogenesis of olfactory sensory neurons Experiment Overall Design: Olfactory epithelium from LIp-C-treated and Lip-O-treated mice was microdissected for RNA extraction and hybridization on Affymetrix microarrays. We compared levels of gene expression in macrophage-depleted and non-depleted sham and 48 hr OBX mice using a 2x2 ANOVA and pairwise comparisons to identify molecular mechanisms of macrophage-mediated neurodegeneration, neuroprotection, and neurogenesis and to validate the gene expression patterns using real-time RT-PCR and immunohistochemistry
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-6540.idf.txt
Sample and data relationshipE-GEOD-6540.sdrf.txt
Raw data (1)E-GEOD-6540.raw.1.zip
Processed data (1)E-GEOD-6540.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-6540.eSet.r
Links