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E-GEOD-65365 - A Nested Parallel Experiment Demonstrates Differences in Intensity-Dependence Between RNA-Seq and Microarrays

Status
Released on 31 January 2015, last updated on 8 February 2015
Organism
Saccharomyces cerevisiae
Samples (32)
Array (1)
Protocols (6)
Description
We carried out a parallel nested experiment performed simultaneously on RNA-Seq and microarrays that systematically split variation into four stages (treatment, biological variation, library preparation, and chip/lane noise), allowing a separation and comparison of the sources of variation in a well-controlled cellular system, Saccharomyces cerevisiae. Nested design. Two conditions (E for ethanol and G for glucose as sole carbon source), each grown on two biological replicates (1 and 2), prepared in two technical preparations (A and B), then hybridized to two chips (1 and 2) This submission represents microarray component of study.
Experiment type
transcription profiling by array 
Contacts
David Garrett Robinson <dgrtwo@princeton.edu>, David G Robinson, Jean Y Wang
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-65365.idf.txt
Sample and data relationshipE-GEOD-65365.sdrf.txt
Raw data (1)E-GEOD-65365.raw.1.zip
Processed data (1)E-GEOD-65365.processed.1.zip
Array designA-GEOD-11382.adf.txt
Links