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E-GEOD-65225 - Genomic Run On (GRO): determination of the nascent transcriptional rates in a series of Hrp1 mutants

Status
Released on 31 December 2015, last updated on 11 January 2016
Organism
Saccharomyces cerevisiae
Samples (6)
Array (1)
Protocols (6)
Description
The goal of the project was to check the differential effect on transcription rate of a sudden degron-induced depletion of Hpr1 protein and its comparison towards the effect of a constitutive absence of Hpr1 protein in a deletion strain. Cells were grown in YP-Raffinose + 0.1 mM CuSO4 at 26ºC to 0.5 OD600. At that cells were spun-down and changed to YP-Galactose without CuSO4 for 30 min at 26ºC. Then cells were shifted to 37ºC to induce degron-mediated proteolysis and recovered after 30 min. Samples include wt, hpr1delta and hpr1-degron after 0 or 30 min growth at 30ºC.
Experiment type
transcription profiling by array 
Contacts
Jose E. Perez-Ortin <jose.e.perez@uv.es>, García-Martínez José, Jordan-Pla Antonio, José E Pérez-Ortín
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-65225.idf.txt
Sample and data relationshipE-GEOD-65225.sdrf.txt
Raw data (1)E-GEOD-65225.raw.1.zip
Processed data (1)E-GEOD-65225.processed.1.zip
Array designA-GEOD-13619.adf.txt
Links