Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-65225 - Genomic Run On (GRO): determination of the nascent transcriptional rates in a series of Hrp1 mutants
Released on 31 December 2015, last updated on 11 January 2016
The goal of the project was to check the differential effect on transcription rate of a sudden degron-induced depletion of Hpr1 protein and its comparison towards the effect of a constitutive absence of Hpr1 protein in a deletion strain. Cells were grown in YP-Raffinose + 0.1 mM CuSO4 at 26ºC to 0.5 OD600. At that cells were spun-down and changed to YP-Galactose without CuSO4 for 30 min at 26ºC. Then cells were shifted to 37ºC to induce degron-mediated proteolysis and recovered after 30 min. Samples include wt, hpr1delta and hpr1-degron after 0 or 30 min growth at 30ºC.
transcription profiling by array
Jose E. Perez-Ortin <firstname.lastname@example.org>, García-Martínez José, Jordan-Pla Antonio, José E Pérez-Ortín