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E-GEOD-65198 - Temporal Changes in Rat Liver Gene Expression after Acute Cadmium and Chromium Exposure

Status
Released on 21 May 2015, last updated on 23 May 2015
Organism
Rattus norvegicus
Samples (59)
Array (1)
Protocols (6)
Description
U.S. Service Members and civilians are at risk of exposure to a variety of environmental health hazards throughout their normal duty activities and in industrial occupations. Metals are widely used in large quantities in a number of industrial processes and are a common environmental toxicant, which increases the possibility of being exposed at toxic levels. While metal toxicity has been widely studied, the exact mechanisms of toxicity remain unclear. In order to further elucidate these mechanisms and identify candidate biomarkers, rats were exposed via a single intraperitoneal injection to three concentrations of CdCl2 and Na2Cr2O7, with livers harvested at 1, 3, or 7 days after exposure. Cd and Cr accumulated in the liver at 1 day post exposure. Cd levels remained elevated over the length of the experiment, while Cr levels declined. Metal exposures induced ROS, including hydroxyl radical (•OH), resulting in DNA strand breaks and lipid peroxidation. Interestingly, ROS and cellular damage appeared to increase with time post-exposure in both metals, despite declines in Cr levels. Differentially expressed genes were identified via microarray analysis. Both metals perturbed gene expression in pathways related to oxidative stress, metabolism, DNA damage, cell cycle, and inflammatory response. This work provides insight into the temporal effects and mechanistic pathways involved in acute metal intoxication, leading to the identification of candidate biomarkers. Rats (5 per group) were exposed via a single intraperitoneal injection to three concentrations of CdCl2, Na2Cr2O7, or vehicle control,with livers harvested at 1, 3, or 7 days after exposure. Only the Cd mid dose and Cr high dose were selected for microarray analysis.
Experiment type
transcription profiling by array 
Contacts
Michael Scott Madejczyk <geo@ncbi.nlm.nih.gov>, Christine E Baer, David A Jackson, John A Lewis, Jonathan D Stallings, Michael S Madejczyk, Stephen S Leonard, Valerie C Minarchick, William E Dennis
Citation
Temporal Changes in Rat Liver Gene Expression after Acute Cadmium and Chromium Exposure. Madejczyk MS, Baer CE, Dennis WE, Minarchick VC, Leonard SS, Jackson DA, Stallings JD, Lewis JA.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-65198.idf.txt
Sample and data relationshipE-GEOD-65198.sdrf.txt
Raw data (1)E-GEOD-65198.raw.1.zip
Processed data (1)E-GEOD-65198.processed.1.zip
Array designA-AFFY-43.adf.txt
Links