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E-GEOD-65042 - Intestinal development in two divergent chicken lines (X and Y)

Status
Released on 14 January 2016, last updated on 15 January 2016
Organism
Gallus gallus
Samples (45)
Array (1)
Protocols (6)
Description
The objective of this study was to investigate the impact of genotypic variation on both early microbial colonization of the gut and functional development of intestinal tissue. From two genetically diverse chicken lines intestinal content samples were taken for microbiota analyzes and intestinal tissue samples were extracted for gene expression analyzes, both at three subsequent time-points (days 0, 4, and 16). 1-day-old chicks (Lines X and Y) were housed in a floor pen system in which the chicks had ad libitum access to feed and water. At days 0, 4 and 16, 80 birds of each line X and line Y were sacrificed for tissue sampling, in total 240 birds per line. Subsequently, samples from these 80 birds were pooled in 8 pools of ten birds.
Experiment type
transcription profiling by array 
Contacts
Dirkjan Schokker <dirkjan.schokker@wur.nl>, J M Rebel, M A Smits, S A Vastenhouw
Citation
Early life microbial colonization of the gut and intestinal development differ between genetically divergent broiler lines. Schokker D, Veninga G, Vastenhouw SA, Bossers A, de Bree FM, Kaal-Lansbergen LM, Rebel JM, Smits MA. , PMID:26017153
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-65042.idf.txt
Sample and data relationshipE-GEOD-65042.sdrf.txt
Raw data (1)E-GEOD-65042.raw.1.zip
Processed data (1)E-GEOD-65042.processed.1.zip
Array designA-GEOD-18890.adf.txt
Links