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E-GEOD-64622 - Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [RNA-seq]

Status
Released on 31 July 2015, last updated on 19 August 2015
Organism
Mus musculus
Samples (8)
Protocols (3)
Description
MyoD and NeuroD2 are master regulators of myogenesis and neurogenesis and bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage-specification, we generated a MyoD-mutant with the DNA binding specificity of NeuroD2. Our results demonstrate that redirecting MyoD binding from MyoD-private sites to NeuroD2-private sites, despite preserved binding to the MyoD/NeuroD2-shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis. RNA-seq profiling of mouse P19 cells transfected with MyoD, NeuroD2 and chimera mutants. The chimeric mutants are MyoD with the bHLH domain replaced with the NeuroD2 bHLH domain.
Experiment type
RNA-seq of coding RNA 
Contacts
Zizhen yao <yzizhen@fhcrc.org>, Abraham Fong, Jun W Zhong, Stephen Tapscott, Zizhen Yao
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-64622.idf.txt
Sample and data relationshipE-GEOD-64622.sdrf.txt
Additional data (1)E-GEOD-64622.additional.1.zip
Links