E-GEOD-6432 - Transcription profiling of human fibroblasts grown in petri dishes vs. attached to a tissue engineering scaffold sampled at 7 timepoints
Submitted on 4 December 2006, released on 26 October 2007, last updated on 27 March 2012
A goal of tissue engineering is to produce a scaffold material that will guide cells to differentiate and regenerate functional replacement tissue at the site of injury. Little is known about how cells respond on a molecular level to tissue engineering scaffold materials. In this work we used oligonucleotide microarrays to interrogate gene expression profiles associated with cell-biomaterial interactions. We seeded collagen-glycosaminoglycan meshes, a widely used tissue engineering scaffold material, with human IMR-90 fibroblasts and compared transcript levels with control cells grown on tissue culture polystyrene. Genes involved in cell signaling, extracellular matrix remodeling, inflammation, angiogenesis and hypoxia were all activated in cells on the collagen-GAG mesh. Understanding the impact of a scaffold on attached cells will facilitate the design of improved tissue engineering materials.
transcription profiling by array, co-expression, growth condition, time series
Global gene expression of cells attached to a tissue engineering scaffold. Catherine M Klapperich, Carolyn R Bertozzi. Biomaterials 25(25):5631-41 (2004)