15 protocols
AccessionType
feature_extraction
Title: Affymetrix CEL analysis. Description:
hybridization
We used the Affymetrix Hybridization Oven 640 and GeneChipᅢツᅡᆴ Fluidics Station 450 according to the manufacturer's recomended protocols.
labeling
We used GeneChipᅢツᅡᆴ One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
hybridization
We used the Affymetrix Hybridization Oven 640 and GeneChipᅢツᅡᆴ Fluidics Station 450 according to the manufacturer's recommended protocols.
labeling
We used GeneChipᅢツᅡᆴ One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
nucleic_acid_extraction
We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
grow
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
specified_biomaterial_action
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
nucleic_acid_extraction
We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
grow
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
specified_biomaterial_action
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
grow
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
specified_biomaterial_action
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
grow
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
specified_biomaterial_action
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.