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E-GEOD-63877 - Microarray analyses of ESAM positive cells in the CD34(+) CD38(-) fraction of collagenase-treated bones

Status
Released on 5 December 2014, last updated on 2 January 2015
Organism
Homo sapiens
Samples (2)
Array (1)
Protocols (6)
Description
We previously identified endothelial cell-selective adhesion molecule (ESAM) as a novel functional marker of hematopoietic stem cells (HSCs) in mice. To examine ESAM expression in human, we analyzed diverse HSC sources using flow cytometry. From mononuclear cells of collagenase-treated human hip bones, we obtained two ESAM positive populations in CD34(+) CD38(-) fraction, referred to as ESAM(High) and ESAM(Bright). Then we conducted microarray analyses comparing gene expression signatures between these two populations. Trabecular tissues of the femora were treated with collagenase IV and DNase. Mononuclear cells were collected, and CD34(+) CD38(-) ESAM(High) or ESAM(Bright) cells were sorted.
Experiment type
transcription profiling by array 
Contacts
Michiko Ichii, Takafumi Yokota, Tomohiko Ishibashi
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-63877.idf.txt
Sample and data relationshipE-GEOD-63877.sdrf.txt
Raw data (1)E-GEOD-63877.raw.1.zip
Processed data (1)E-GEOD-63877.processed.1.zip
Array designA-GEOD-16699.adf.txt
Links