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E-GEOD-63800 - The Sm protein methyltransferase PRMT5, is not required for primordial germ cell specification in mice

Status
Released on 5 December 2014, last updated on 2 January 2015
Organism
Mus musculus
Samples (8)
Protocols (3)
Description
PRMT5 is a type II protein arginine methyltransferase with roles in stem cell biology, reprogramming, cancer and neurogenesis. During embryogenesis in the mouse it was hypothesized that PRMT5 functions with the master germline determinant BLIMP1 to promote primordial germ cell (PGC) specification. Using a Blimp1-Cre germline conditional knockout, we discovered that Prmt5 has no major role in murine germline specification, or the first global epigenetic reprogramming event involving depletion of cytosine methylation from DNA and histone H3 lysine 9 dimethylation from chromatin. Instead, we discovered that PRMT5 functions at the conclusion of PGC reprogramming I to promote proliferation, survival and expression of the gonadal germline program as marked by MVH. We show that PRMT5 regulates gene expression by promoting methylation of the Sm spliceosomal proteins, and significantly altering the spliced repertoire of RNAs in mammalian embryonic cells and primordial cells. Examination of transcriptional profile of iPHet (Control) vs. iPKO (Prmt5 knock out) 2i Embryonic Stem Cells
Experiment type
RNA-seq of coding RNA 
Contacts
Amander T Clark, Juehua Yu, Ziwei Li
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-63800.idf.txt
Sample and data relationshipE-GEOD-63800.sdrf.txt
Processed data (1)E-GEOD-63800.processed.1.zip
Links