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E-GEOD-63703 - Short and Long RNA sequencing of human mature erythrocytes

Status
Released on 19 November 2015, last updated on 30 November 2015
Organism
Homo sapiens
Samples (13)
Protocols (6)
Description
We employed high-throughput sequencing of both short (~18-24nt) and long (>200nt) RNAs in human erythrocytes. We obtained blood from five healthy individuals for the short (small) RNA-seq library preparations and blood from three individuals for the long RNA-seq library preparations. We identified an abundant, diverse population of RNAs. Both polyadenylated and nonpolyadenlated long RNAs were identified. Additionally, known and novel microRNAs were identified in the short RNA dataset using the probabalistic modeling algorithm miRDeep. These RNAs lend insight into erythrocyte biology and provide utility as potential biomarkers. To determine both shared and unique aspects of the erythrocyte long RNA transcriptome, we compared this transcriptome with that of the PBMC and CD34+ erythroid progenitor transcriptomes. Sequencing of RNAs from mature erythrocytes of healthy individuals
Experiment types
RNA-seq of coding RNA, RNA-seq of non coding RNA 
Contacts
Jen-Tsan Ashley Chi <geo@ncbi.nlm.nih.gov>, David L Corcoran, Dereje D Jima, Jennifer F Doss
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-63703.idf.txt
Sample and data relationshipE-GEOD-63703.sdrf.txt
Additional data (1)E-GEOD-63703.additional.1.zip
Links