Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-63703 - Short and Long RNA sequencing of human mature erythrocytes
Released on 19 November 2015, last updated on 30 November 2015
We employed high-throughput sequencing of both short (~18-24nt) and long (>200nt) RNAs in human erythrocytes. We obtained blood from five healthy individuals for the short (small) RNA-seq library preparations and blood from three individuals for the long RNA-seq library preparations. We identified an abundant, diverse population of RNAs. Both polyadenylated and nonpolyadenlated long RNAs were identified. Additionally, known and novel microRNAs were identified in the short RNA dataset using the probabalistic modeling algorithm miRDeep. These RNAs lend insight into erythrocyte biology and provide utility as potential biomarkers. To determine both shared and unique aspects of the erythrocyte long RNA transcriptome, we compared this transcriptome with that of the PBMC and CD34+ erythroid progenitor transcriptomes. Sequencing of RNAs from mature erythrocytes of healthy individuals
RNA-seq of coding RNA, RNA-seq of non coding RNA
Jen-Tsan Ashley Chi <email@example.com>, David L Corcoran, Dereje D Jima, Jennifer F Doss
A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytes. Doss JF, Corcoran DL, Jima DD, Telen MJ, Dave SS, Chi JT. , PMID:26573221