2 protocols
nucleic acid extraction protocol
48 hours post-transfection, cells were UV cross-linked and prepared as described previously (Chi SW, et al. 2009, Nature 460:479). Samples were prepared for sequencing as described previously (Licatalosi D, et al. 2008, Nature 456:464-U22).
growth protocol
Cells were grown in DMEM-based media supplemented with 10% FBS, 1% L-glutamine, 1% Sodium Pyruvate, and 1% Pen/Strep