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E-GEOD-63371 - Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-TAB-seq

Status
Released on 11 July 2015, last updated on 19 August 2015
Organism
Caldicellulosiruptor kristjanssonii, synthetic construct
Samples (7)
Protocols (4)
Description
N4-methylcytosine is a major DNA modification integral to restriction-modification (R-M) systems in bacterial genomes. Here we describe 4mC-Tet-Assisted Bisulfite-sequencing (4mC-TAB-seq), a method that accurately and rapidly reveals the genome-wide locations of N4-methylcytosines at single-base resolution. By coupling Tet-mediated oxidation with a modified sodium bisulfite conversion reaction, unmodified cytosines and 5-methylcytosines are read out as thymines, whereas N4-methylcytosines are read out as cytosines revealing their positions throughout the genome. 4mC-TAB-seq
Experiment type
methylation profiling by high throughput sequencing 
Contacts
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-63371.idf.txt
Sample and data relationshipE-GEOD-63371.sdrf.txt
Processed data (1)E-GEOD-63371.processed.1.zip
Links