Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-63189 - Identification and functional characterization of long noncoding RNAs in breast cancer

Status
Released on 30 July 2015, last updated on 19 August 2015
Organism
Homo sapiens
Samples (25)
Protocols (4)
Description
In this study, we have integrated RNA-seq data from subcellular fractionated RNA (i.e., cytoplasm, nucleoplasm, and chromatin-associated) with GRO-seq data using a novel bioinformatics pipeline. This has yielded a comprehensive catalog of polyadenylated lncRNAs in MCF-7 cells, about half of which have not been annotated previously and about a quarter of which are estrogen-regulated. Knockdown of selected lncRNAs, such as lncRNA152 and lncRNA67 followed by RNA-seq suggest that these lncRNAs regulate the expression of cell cycle genes. characterization of long noncoding RNAs
Experiment type
RNA-seq of coding RNA 
Contacts
W. Lee Kraus <lee.kraus@utsouthwestern.edu>, Dae S Kim, Miao Sun, Shrikanth S Gadad, W L Kraus
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-63189.idf.txt
Sample and data relationshipE-GEOD-63189.sdrf.txt
Additional data (1)E-GEOD-63189.additional.1.zip
Links