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E-GEOD-62964 - Gene expression profiling of murine WT and DNR Tregs

Status
Released on 5 November 2014, last updated on 8 November 2014
Organism
Mus musculus
Samples (2)
Array (1)
Protocols (6)
Description
Donminant negative transform growth factor receptor II (DNR) mice were served as a murine primary biliary cirrhrosis model. CD4+Foxp3+ Regulatory T cells (Tregs) play a critical role in self-tolerance and in regulating PBC. In order to determine whether DNR mice derived Tregs processed defective function compared with WT Tregs, CD4+Foxp3+ Treg cells were sorted from DNR and WT mice, respectively, then gene expression analysis was performed by using the Affymetrix GeneChip Mouse Genome 430 2.0 arrays CD4+Foxp3+ Tregs were sorted from the spleen of 10-week-old DNR mice and B6 wild-type mice, respectively. RNA of each sample was then extracted and hybridized on Affymetrix microarrays to detail differences between DNR Tregs and WT Tregs in gene expression.
Experiment type
transcription profiling by array 
Contacts
Wei Yang, Yin-Hu Wang, Zhe-Xiong Lian
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-62964.idf.txt
Sample and data relationshipE-GEOD-62964.sdrf.txt
Raw data (1)E-GEOD-62964.raw.1.zip
Processed data (1)E-GEOD-62964.processed.1.zip
Array designA-AFFY-45.adf.txt
Links