E-GEOD-6282 - C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria

Status
Released on 17 November 2006, last updated on 3 June 2014
Organism
Caenorhabditis elegans
Samples (1)
Protocols (6)
Description
RNA interference (RNAi) is a phylogenetically widespread gene silencing process triggered by doublestranded RNA (dsRNA). In plants and C. elegans, two distinct populations of small RNAs have been proposed to participate in RNAi : "Primary siRNAs" (derived from Dicer nuclease-mediated cleavage of the original trigger) and "Secondary siRNAs" (additional small RNAs whose synthesis requires an RNA-directed RNA polymerase [RdRP]). Analyzing small RNAs associated with ongoing RNAi in C. elegans, we found secondary siRNAs to comprise the vast majority. The bulk of secondary siRNAs exhibited structure and sequence indicative of a biosynthetic mode where each molecule derives from an independent de novo initiation by RdRP. Analysis of endogenous small RNAs indicated that a fraction derive from a biosynthetic mechanism that is similar to that of secondary siRNAs formed during RNAi, suggesting that small antisense transcripts derived from cellular mRNAs by RdRP activity may have key roles in cellular regulation. Keywords: C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria
Experiment type
RNA-seq of non coding RNA 
Contacts
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-6282.idf.txt
Sample and data relationshipE-GEOD-6282.sdrf.txt
Raw data (1)E-GEOD-6282.raw.1.zip
Processed data (1)E-GEOD-6282.processed.1.zip
Additional data (1)E-GEOD-6282.additional.1.zip
Links