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E-GEOD-62641 - Comparison of TLR4-stimulated mouse microglia from wildtype and CD14ko strains

Status
Released on 23 October 2015, last updated on 8 December 2015
Organism
Mus musculus
Samples (24)
Protocols (2)
Description
The analysis reveals the importance of CD14 in microglial responses to pathogen- and damage-associated molecular patterns, based on stimulations with lipopolysaccharide (LPS) and fiibronectin (FN). The data show a dose- and agonist-dependent involvement of CD14. Lack of CD14 impairs responses to low-dose challenegs with LPS, whereas high-dose challenegs are affected to a much lesser degree. For FN, CD14 deficiency causes a major impairment of the response capacity. Mouse microglia from wt and cd14-/- strains were stimulated with 0.1 and 10 ng/ml of LPS or with 100 µg/ml of mouse FN for 3 h. Untreated cells served as the controls. Each condition was based on three independent cell preparations as wells a processing.
Experiment type
RNA-seq of coding RNA 
Contacts
Gabriela Salinas-Riester, Hana Janova, Uwe-Karsten Hanisch
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-62641.idf.txt
Sample and data relationshipE-GEOD-62641.sdrf.txt
Additional data (1)E-GEOD-62641.additional.1.zip
Links