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E-GEOD-62315 - Transcription profiling by array of 30-day old detached grape leaves exposed to ultraviolet C radiation for 6 or 12 hours against unexposed controls

Status
Released on 15 November 2014, last updated on 1 May 2015
Organism
Vitis vinifera
Samples (9)
Array (1)
Protocols (7)
Description
Solar ultraviolet C(UV-C)radiation reaching the Earth’s surface is little due to the filtering effects of the stratospheric ozone layer. At present, artificial UV-C irradiation is utilized for different biological processes. Grape is a major fruit crop around the world. Research has shown that UV-C irradiation induced the biosynthesis of phenols. However, changes at the molecular level in response to UV-C and leading to these effects are poorly understood. To elucidate the effect of UV-C on expression of genes in grape and the response mechanism, transcript abundance of grape (Vitis vinifera L.) leaves was quantified using the Affymetrix Grape Genome oligonucleotide microarray (15,700 transcripts) Grape leaves were exposed to UV-C irradiation at 6W/m2 for 10 min. LCK-0-1, LCK-0-2 and LCK-0-3 are 0 h after the initiation of treatment and as the controls; LTR-6-1, LTR-6-2 and LTR-6-3 are for 6 h after the initiation of treatment; LTR-12-1, LTR-12-2 and LTR-12-3 are for 12 h after the initiation of treatment. Three replicates for each time point. 9 samples in all.
Experiment type
transcription profiling by array 
Contacts
Huifen Xi, Lijun Wang
Citation
Transcriptomic Analysis of Grape (Vitis vinifera L.) Leaves after Exposure to Ultraviolet C Irradiation. Huifen Xi, Ling Ma, Guotian Liu, Nian Wang, Junfang Wang, Lina Wang, Zhanwu Dai, Shaohua Li, Lijun Wang.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-62315.idf.txt
Sample and data relationshipE-GEOD-62315.sdrf.txt
Raw data (1)E-GEOD-62315.raw.1.zip
Processed data (1)E-GEOD-62315.processed.1.zip
Additional data (1)E-GEOD-62315.additional.1.zip
Array designA-AFFY-78.adf.txt
Links