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E-GEOD-62053 - S-adenosylmethionine (SAM) changes DNA methylation patterns in prostate cancer cells

Status
Released on 1 March 2015, last updated on 7 March 2015
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (5)
Description
Genome-wide DNA methylation profiling of human PC3 invasive prostate cancer cell line treated with vehicle control (SAH, S-adenosylhomocysteine) and with SAM (S-adenosylmethionine) as well as of untreated human LNCaP non-invasive prostate cancer cell line. The Illumina Infinium 450k Human DNA Methylation BeadChip v1.2 was used to obtain DNA methylation profiles across approximately 450,000 CpGs in human cell lines exposed to described treatments. Samples included biological triplicate of PC3 control (SAH treated), biological triplicate of PC3 treated with SAM, and biological duplicate of LNCaP untreated. Bisulfite-converted DNA from the 8 samples were hybridised to the Illumina Infinium 450k Human Methylation BeadChip v1.2.
Experiment type
methylation profiling by array 
Contacts
Ani Arakelian, Barbara Stefanska, Flora Chik, Moshe Szyf, Nicholas Shukeir, Shafaat A Rabbani, Surabhi Parashar
Citation
Pharmacological methyl group donors block skeletal metastasis in vitro and in vivo. Shukeir N, Stefanska B, Parashar S, Chik F, Arakelian A, Szyf M, Rabbani SA. , PMID:25631332
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-62053.idf.txt
Sample and data relationshipE-GEOD-62053.sdrf.txt
Raw data (1)E-GEOD-62053.raw.1.zip
Processed data (1)E-GEOD-62053.processed.1.zip
Array designA-GEOD-13534.adf.txt
Links