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E-GEOD-61898 - Polysome profiling in CIRCADIAN CLOCK ASSOCIATED 1-overexpressing (CCA1-ox) Arabidopsis thaliana over a 24-hour diurnal cycle

Status
Released on 1 February 2015, last updated on 8 February 2015
Organism
Arabidopsis thaliana
Samples (36)
Array (1)
Protocols (6)
Description
Circadian control of gene expression has been established in plants at the transcriptional level, but relatively little is known about circadian control of translation. We used polysome profiling to characterize regulation of transcription and translation over a 24-hour diurnal cycle in Arabidopsis, both in wild type and in plants with a disrupted clock due to constitutive overexpression of the CIRCADIAN CLOCK ASSOCIATED 1 gene (CCA1-ox, AGI AT2G46830). 10 day-old wild type and CCA1-ox (described in Cell. 1998 Jun 26;93(7):1207-17) Arabidopsis seedlings were harvested at 6am (Zeitgeber time ZT0), 12pm (ZT6), 6pm (ZT12), and 12am (ZT18), with 3 replicates for each time and genotype.
Experiment type
transcription profiling by array 
Contacts
Albrecht G von Arnim <vonarnim@utk.edu>, Albrecht von Arnim, Anamika Missra, Ben Ernest
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-61898.idf.txt
Sample and data relationshipE-GEOD-61898.sdrf.txt
Raw data (1)E-GEOD-61898.raw.1.zip
Processed data (1)E-GEOD-61898.processed.1.zip
Array designA-AFFY-2.adf.txt
Links