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E-GEOD-61471 - Cooperativity, specificity and evolutionary stability of Polycomb targeting in Drosophila

Status
Released on 20 October 2014, last updated on 25 October 2014
Organism
Drosophila melanogaster
Samples (2)
Protocols (2)
Description
We report the evolutionary behaviour of Polycomb group proteins, their recruitment factors and their underlying sequences by performing ChIP-seq analysis in 4-5 different Drosophila species (GSE60428) and HiC analysis in Drosophila melanogaster. We demonstrate an extremely high conservation of Polycomb repressive domains across Drosophila species We validate few cases of PRE divergence that shows that cis-driven PRE evolution is a rare event. We further show that PHO recruitment to Polycomb domains is evolutionarily robust to motif changes and that PRC1 stabilizes binding of its key recruiter HiC experiments in wild type drosophila embryos
Experiment type
other 
Contacts
Jia-Ming Chang <chang.jiaming@gmail.com>, Amos Tanay, Aubin Thomas, Bernd Schuettengruber, Eitan Yaffe, Giacomo Cavalli, Hugues Parrinello, Marianne Entrevan, Noa O Elkayam, Shani Stern, Tom Sexton
Citation
Cooperativity, specificity, and evolutionary stability of polycomb targeting in Drosophila. Schuettengruber B, Oded Elkayam N, Sexton T, Entrevan M, Stern S, Thomas A, Yaffe E, Parrinello H, Tanay A, Cavalli G. , PMID:25284790
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-61471.idf.txt
Sample and data relationshipE-GEOD-61471.sdrf.txt
Additional data (2)E-GEOD-61471.additional.1.zip, E-GEOD-61471.additional.2.zip
Links