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E-GEOD-6125 - Transcription profiling of rice virus-susceptible and -resistant lines (TW16-1263 and -1029)

Status
Submitted on 25 October 2006, released on 14 June 2008, last updated on 2 May 2014
Organism
Oryza sativa
Samples (12)
Array (1)
Protocols (9)
Description
Near-isogenic lines TW16-1263 and TW16-1029 were developed from the cross between TN1 (recurrent parent susceptible to rice tungro spherical virus (RTSV)) and Utri Merah (donor parent resistant to RTSV). TW16-1263 is susceptible to RTSV, whereas TW16-1029 is resistant to RTSV. In order to identify genes which are constitutively differentially expressed between TW16-1263 and TW16-1029, the gene expression in the two lines were compared at three different conditions (healthy, green leafhopper (GLH)-inoculated, and RTSV-inoculated). Experiment Overall Design: Total RNA was isolated from 14-day old plants of TW16-1263 and TW16-1029 at three different conditions (healthy, GLH-inoculated, and RTSV-inoculated). The levels of gene expression in the two lines of the same condition were compared by microarray. Each experiment was repeated twice.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-6125.idf.txt
Sample and data relationshipE-GEOD-6125.sdrf.txt
Processed data (1)E-GEOD-6125.processed.1.zip
Array designA-AGIL-10.adf.txt
Links