Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-61057 - Transcription profiling by high throughput sequencing of aleurone and starchy endosperm cells were isolated by laser-capture microdissection in maize nkd mutants

Status
Released on 28 January 2015, last updated on 24 February 2016
Organism
Zea mays
Samples (12)
Protocols (4)
Description
The objective of the current study is to unravel the gene regulatory networks controlled by the nkd genes during maize endosperm development. We compared wild type (B73) vs. nkd mutant (introgressed into B73 background) transcriptomes in aleurone vs. starchy endosperm cell types captured by laser capture microdissection technology. We performed RNA seq analysis of mid-mature (15DAP) endosperm in two cell types [aleurone (A) and starchy endosperm (S)] of wild type B73 (B) and nkd mutant (N) kernels with three independent biological replicates.
Experiment type
RNA-seq of coding RNA 
Contacts
Philip W Becraft <geo@ncbi.nlm.nih.gov>, Anjanasree K Neelakandan, Bryan Gontarek, Erik Vollbrecht, Gibum Yi
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-61057.idf.txt
Sample and data relationshipE-GEOD-61057.sdrf.txt
Additional data (1)E-GEOD-61057.additional.1.zip
Links