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E-GEOD-61057 - Transcription profiling by high throughput sequencing of aleurone and starchy endosperm cells were isolated by laser-capture microdissection in maize nkd mutants

Released on 28 January 2015, last updated on 24 February 2016
Zea mays
Samples (12)
Protocols (4)
The objective of the current study is to unravel the gene regulatory networks controlled by the nkd genes during maize endosperm development. We compared wild type (B73) vs. nkd mutant (introgressed into B73 background) transcriptomes in aleurone vs. starchy endosperm cell types captured by laser capture microdissection technology. We performed RNA seq analysis of mid-mature (15DAP) endosperm in two cell types [aleurone (A) and starchy endosperm (S)] of wild type B73 (B) and nkd mutant (N) kernels with three independent biological replicates.
Experiment type
RNA-seq of coding RNA 
Philip W Becraft <>, Anjanasree K Neelakandan, Bryan Gontarek, Erik Vollbrecht, Gibum Yi
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-61057.idf.txt
Sample and data relationshipE-GEOD-61057.sdrf.txt
Additional data (1)