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E-GEOD-60835 - Transcription profiling by high throughput sequencing of Arabidopsis wild type, det1-1 mutant, and pif quadruple mutant seedlings grown in the dark and of wild type seedlings exposed to white light for 6 hours

Status
Released on 19 March 2015, last updated on 16 February 2016
Organism
Arabidopsis thaliana
Samples (12)
Protocols (4)
Description
Arabidopsis seedlings undergo photomorphogenic development even in darkness when the function of De-etiolated 1 (DET1), a repressor of photomorphogenesis, is disrupted. Our results indicate that DET1 directly interacts with a group of transcription factors known as the phytochrome-interacting factors (PIFs). Furthermore, our results suggest that DET1 positively regulates PIF protein levels primarily by stabilizing PIF proteins in the dark. Genomic analysis also revealed that DET1 may control the expression of light-regulated genes to mediate photomorphogenesis partially through PIFs. Total of twelve samples, two treatments and three genotypes, and each have three replicates.
Experiment type
RNA-seq of coding RNA 
Contacts
Xu Zhao Gao <gzhx1989@163.com>, Dafang Tang, Hang He, Haodong Chen, Jie Dong, Kunlun Li, Renbo Yu, William Terzaghi, Xing W Deng, Zhaoxu Gao
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-60835.idf.txt
Sample and data relationshipE-GEOD-60835.sdrf.txt
Additional data (1)E-GEOD-60835.additional.1.zip
Links