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E-GEOD-60568 - Disruption of transkingdom communication by antibiotics leads to immune and mitochondrial dysfunction in the gut

Status
Released on 26 January 2015, last updated on 3 February 2015
Organism
Mus musculus
Samples (190)
Array (1)
Protocols (11)
Description
We analyzed the effects of antibiotics using a popular model of gut microbiota depletion in mice by a cocktail of antibiotics. We combined intestinal transcriptome together with metagenomic analysis of the gut microbiota to develop a new bioinformatics approach that probes the links between microbial components and host functions. We found that most antibiotic-induced alterations can be explained by three factors: depletion of the microbiota; direct effects of antibiotics on host tissues; and the effects of remaining antibiotic-resistant microbes. While microbe depletion led to down-regulation of immunity, the two other factors primarily inhibited mitochondrial gene expression and amounts of active mitochondria, and induced cell death. By reconstructing and analyzing a transkingdom network, we discovered that these toxic effects were mediated by virulence/quorum sensing in antibiotic-resistant bacteria. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
Experiment type
transcription profiling by array 
Contact
Citation
Uncovering effects of antibiotics on the host and microbiota using transkingdom gene networks. Morgun A, Dzutsev A, Dong X, Greer RL, Sexton DJ, Ravel J, Schuster M, Hsiao W, Matzinger P, Shulzhenko N. , PMID:25614621
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-60568.idf.txt
Sample and data relationshipE-GEOD-60568.sdrf.txt
Raw data (1)E-GEOD-60568.raw.1.zip
Processed data (1)E-GEOD-60568.processed.1.zip
Array designA-GEOD-9354.adf.txt
Links