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E-GEOD-60551 - Transcription profiling by array of HaCat cells with or without over-expression of BCL-3

Status
Released on 4 November 2014, last updated on 30 April 2015
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
Constitutive activation of EGFR- and NF-kB-dependent pathways is a hallmark of cancer, yet signaling proteins that connect both oncogenic cascades are poorly characterized. Here we define KIAA1199 as a BCL-3- and p65-dependent gene in transformed keratinocytes. KIAA1199 expression is enhanced upon human papillomavirus (HPV) infection and is aberrantly expressed in clinical cases of cervical (pre)neoplastic lesions. Mechanistically, KIAA1199 binds Plexin A2 and protects from Semaphorin 3A-mediated cell death by promoting EGFR stability and signaling. Moreover, KIAA1199 is an EGFR-binding protein and KIAA1199 deficiency impairs EGF-dependent Src, MEK1 and ERK1/2 phosphorylations. Therefore, EGFR stability and signaling to downstream kinases requires KIAA1199. As such, KIAA1199 promotes EGF-mediated epithelial-mesenchymal transition (EMT). Taken together, our data define KIAA1199 as an oncogenic protein induced by HPV infection and constitutive NF-kB activity that transmits pro-survival and invasive signals through EGFR signaling. We used microarrays to detail the global programme of gene expression upon BCL-3 overexpression We used two experimental conditions, namely HaCat cells infected with a control lentivirus as well as HaCat cells infected with a BCL-3 expressing construct. Both experimental conditions were in triplicates.
Experiment type
transcription profiling by array 
Contacts
Alain Chariot, Benoît Hennuy, Xin Zhang
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-60551.idf.txt
Sample and data relationshipE-GEOD-60551.sdrf.txt
Raw data (1)E-GEOD-60551.raw.1.zip
Processed data (1)E-GEOD-60551.processed.1.zip
Additional data (1)E-GEOD-60551.additional.1.zip
Array designA-AFFY-37.adf.txt
Links