normalization data transformation protocol
Signal intensities of genes were normalized by the quantile method. ID_REF = VALUE = Quantile normalization, median subtraction
array scanning protocol
Arrays were scanned with an Agilent DNA Microarray Scanner.
The labelled RNA were hybridized to a microarray (Agilent Rat whole genome 44K, Agilent Technologies, USA) containing approximately 44,000 probes (~26,600 unique genes) in accordance with the manufacturer's instructions.
The pooled RNA was amplified and labeled using Agilent’s Low RNA Input Linear Amplification kit PLUS (Agilent Technologies, USA).
The mice were housed in a room with the temperature controlled at 24±1 °C at a relative humidity of 50±10%, with a 12h dark/light cycle. Food and water were provided ad libitum throughout the experiment.
sample treatment protocol
During irradiation, the mice were anesthetized with an intraperitoneally administered mixture of 30 mg/kg of zoletil and 10 mg/kg of rompun.
nucleic acid extraction protocol
Total RNA from mouse lung tissues was prepared using the Easy-SpinTM total RNA extraction kit according to the manufacturer’s instructions (iNtRON Biotechnology, Seoul, Republic of Korea).